experimental error biuret test Elkhart Texas

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experimental error biuret test Elkhart, Texas

Colorimetric procedures are routinely used for detecting and measuring a wide variety of substances in blood, urine, water samples, and other fluids of interest to biologists, chemists, doctors, and others. before use. Add your answer Source Submit Cancel Report Abuse I think this question violates the Community Guidelines Chat or rant, adult content, spam, insulting other members,show more I think this question violates I can't think of many examples of experimental errors that could've occured.

The biuret is a good general protein assay for batches of material for which yield is not a problem. Copyright and Intended Use Visitors: to ensure that your message is not mistaken for SPAM, please include the acronym "Bios211" in the subject line of e-mail communications Created by David R. Problem?Pipet falls out of pump? Proteins differ from each other according to the type, number and sequence of amino acids that make up the polypeptide backbone.

After 10 minutes, they are added with 0.25 mL of Lowry reagent 2 and mix them well immediately. 5. Always be watchful for sources of error and minimize them. 6. b. There are chemical tests for simple carbohydrates, for proteins, for steroids, and so on.

Wear splash goggles and gloves. 2. Wipe the surface dry/clean. 2. a. This tiny source of error will be the same for all of your tubes.

Error is always present to some degree when experimental procedures are being done and when measurements are being made. Click to set custom HTML Brilliant Biology Student 2016 Create a free website Powered by Create your own free website Start your own free website A surprisingly easy drag & drop The results supposed to be vice versa, omega 3 should contain the most protein whereas chicken 1 the least. Next, dispense the 0.5 MKCl solution into all tubes that are supposed to get it.

The line probably won't go through some points. You are responsible for understanding all of the material (theory, procedures,calculations, plotting of data). Add 1 cm3 of sodium hydroxide solution (40% or bench solution) and 1% copper (II) sulphate solution dropwise (one drop at a time) to the food sampleRepeat the steps above with It is named for the biochemistOliver H.

This is much the same as you did last week except that you'll use only one wavelength today. 1. The pilot lamp should glow, and you may hear the gentle sound of the cooling fan inside. Use the wavelength control knob (on top) to select the desired wavelength; the wavelength values shown in the wavelength dial display window are graduated in nanometers (nm). Macromolecules in Action!Search this site HomeAbstractIntroductionMethodologyResultsDiscussionCitations The Macromolecule Table of ContentsHomeAbstractIntroduction Discussion Post Lab Analysis1.

As mentioned earlier, the 5 points should be in a straight line, but the various kinds of error that occur in doing the work will cause some points to be less Wherever the needle stops, use the light control knob (right front) to move the needle to 100 %T on the scale (= 0 absorbance). Although the molecule,biuret, itself, is of very little significance, there is a group of very important molecules, the proteins, which react with copper sulfate andNaOH the same way thatbiuret reacts. I'm doing a lab in Biology and it asks for examples of eperimental errors found in the lab.

The other 3mL wasbiuret reagent. You will set up a series of test tubes in which you will prepare a series of solutions that differ in protein concentration. After adding NaOH, then add some drops of Cu(II)SO4.I had done all of this, but why can't I get the correct result? Note that the blank solution today is more than just water; in last week's exercise, the blank was only water.

What facts support your answer in Question 1 of the Post Lab Analysis?    Based on the table on the Results page, we can see that 5 out of 5 solutions tested This reduces the probability of breaking thepipet. You will recordaborbance values and %T values for each solution. Determine concentrations of original samples from the amount protein, volume/sample, and dilution factor, if any.

Use the squeeze water bottle and 1-quart RINSE cup to carefully rinse the colorimeter tube with water, drain it briefly,wipe the surface dry/clean. Standard Protein Standard protein (mg/ml) Absorbance (nm) 1 0.155 2 0.193 3 0.210 4 0.273 5 0.317 6 0.357 2. Expand» Details Details Existing questions More Tell us some more Upload in Progress Upload failed. Still having trouble?

Proteins with an abnormally high or low percentage of amino acids with aromatic side groups will give high or low readings, respectively. What you learn in this exercise and in the previous one has many applications, far beyond these exercises. The diluted protein sample is then diluted for another 10% by mixing 1 ml diluted protein sample from the first dilution with 9ml of distilled water. BSA is the reference protein in this work.

please help :(. If a type of molecule has a distinctive color naturally, as riboflavin does, it may be analyzedcolorimetrically as it is, without need for special treatment beforehand. Insert the colorimeter tube into the sample holder (tube label facing front); close the lid. (Don't turn any knobs.) 3. Think about what this means: **** At this moment 540 nm light is passing through the solution and the amount that strikes the detector is registering on the scale you see.

Moisten the outer surface of thepipet end with a tiny drop of water to get a better seal and reinsert thepipet. Slater, RJ (ed.). Usingpipet pumps. and give examples if possible...?

In order to quantitatively determine how much protein is represented by a particular absorbance reading it is necessary to construct a standard curve. QuantitativeColorimetry and theBiuret Test [Be sure to print the Experimental Setup Table also; you must have it to do the exercise.] In the previous lab exercise you learned (a) how theSpectronic Disposal of test tube solutions. The reading of colour at 750 nm for Lowry Assay is recommended because at this wavelength, few other substances absorb light.

They do most of the work in a cell. How might you avoid such error if you were to repeat this lab?    If we were to repeat this lab, one way to avoid the errors listed above is to understand However, most types of organic molecules are colorless in solution; therefore, in order to detect them and measure them in solutions, they must first be made "visible" to the colorimeter. Layne, E.

This requires solving a simple dilution problem, which involves dimensional analysis again. Because no one reagent can be considered the ideal or best protein assay method for all circumstances, most researchers have more than one type of protein assay available in their laboratories. Then you will use the standard curve to estimate the concentration of two albumin "unknown" solutions. This graph is called the standard curve for assay, and can be used to convert the absorbance readings for the experimental samples (eggs from duck, chicken 1 (ayam biasa), chicken 2

Logged deadally Regular Member Mole Snacks: +1/-2 Offline Posts: 15 I'm a mole! Be sure to print it and bring it to lab also. Clean your work area. 7. I'm aware that heavy metals (IE Copper) have a propensity to precipitate protein out of solution, which might account for the cloudiness.Or it might just be an experimental error Logged yoyowong007